2024

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches 
Joshua A Welsh, Deborah CI Goberdhan, Lorraine O'Driscoll, Edit I Buzas, Cherie Blenkiron, Benedetta Bussolati, Houjian Cai, Dolores Di Vizio, Tom AP Driedonks, Uta Erdbrügger, Juan M Falcon‐Perez, Qing‐Ling Fu, Andrew F Hill, Metka Lenassi, Sai Kiang Lim, Mỹ G Mahoney, Sujata Mohanty, Andreas Möller, Rienk Nieuwland, Takahiro Ochiya, Susmita Sahoo, Ana C Torrecilhas, Lei Zheng, Andries Zijlstra, Sarah Abuelreich, Reem Bagabas, Paolo Bergese, Esther M Bridges, Marco Brucale, Dylan Burger, Randy P Carney, Emanuele Cocucci, Rossella Crescitelli, Edveena Hanser, Adrian L Harris, Norman J Haughey, An Hendrix, Alexander R Ivanov, Tijana Jovanovic‐Talisman, Nicole A Kruh‐Garcia, Vroniqa Ku'ulei‐Lyn Faustino, Diego Kyburz, Cecilia Lässer, Kathleen M Lennon, Jan Lötvall, Adam L Maddox, Elena S Martens‐Uzunova, Rachel R Mizenko, Lauren A Newman, Andrea Ridolfi, Eva Rohde, Tatu Rojalin, Andrew Rowland, Andras Saftics, Ursula S Sandau, Julie A Saugstad, Faezeh Shekari, Simon Swift, Dmitry Ter‐Ovanesyan, Juan P Tosar, Zivile Useckaite, Francesco Valle, Zoltan Varga, Edwin van der Pol, Martijn JC van Herwijnen, Marca HM Wauben, Ann M Wehman, Sarah Williams, Andrea Zendrini, Alan J Zimmerman, MISEV Consortium, Sarah Abuelreich, Samar Ahmad, Dina AK Ahmed, Sarah H Ahmed, Elena Aikawa, Naveed Akbar, Kazunari Akiyoshi, David P Al‐Adra, Maimonah E Al‐Masawa, Manuel Albanese, Ainhoa Alberro, María José Alcaraz, Jen Alexander‐Brett, Kimberley L Alexander, Nilufar Ali, Faisal J Alibhai, Susann Allelein, Mark C Allenby, Fausto Almeida, Luis Pereira de Almeida, Sameh W Almousa, Nihal Altan‐Bonnet, Wanessa F Altei, Gloria Alvarez‐Llamas, Cora L Alvarez, Hyo Jung An, Krishnan Anand, Samir EL Andaloussi, Johnathon D Anderson, Ramaroson Andriantsitohaina, Khairul I Ansari, Achille Anselmo, Anna Antoniou, Farrukh Aqil, Tanina Arab, Fabienne Archer, Syrine Arif, David A Armstrong, Onno J Arntz, Pierre Arsène, Luis Arteaga‐Blanco, Nandini Asokan, Trude Aspelin, Georgia K Atkin‐Smith, Dimitri Aubert, Kanchana K Ayyar, Maryam Azlan, Ioannis Azoidis, Anaïs Bécot, Jean‐Marie Bach, Daniel Bachurski, Seoyoon Bae, Reem Bagabas, Roger Olofsson Bagge, Monika Baj‐Krzyworzeka, Leonora Balaj, Carolina Balbi, Bas WM van Balkom, Abhijna R Ballal, Afsareen Bano, Sébastien Banzet, Yonis Bare, Lucio Barile, Bahnisikha Barman, Isabel Barranco, Valeria Barreca, Geneviève Bart, Natasha S Barteneva, Manuela Basso, Mona Batish, Natalie R Bauer, Amy A Baxter, Wilfried W Bazié, Erica Bazzan, Joel EJ Beaumont, Mary Bebawy, Maarten P Bebelman, Apolonija Bedina‐Zavec, Danielle J Beetler, Journal of Extracellular Vesicles 13 (2), e12404 

2023

Single-cell transcriptomics reveal extracellular vesicles secretion with a cardiomyocyte proteostasis signature during pathological remodeling, E Schoger, F Bleckwedel, G Germena, C Rocha, P Tucholla, I Sobitov, W Möbius, M Sitte, C Lenz, M Samak, R Hinkel, ZV Varga, Z Giricz, G Salinas, JC Gross, LC Zelarayán , Communications Biology 6 (1), 79 |  | 2023 

Kinesin-mediated transport in the secretion of extracellular vesicles
H Tietz, JC Gross Trillium Extracellular Vesicles 1 (5), https://doi.org/10.47184/tev.2023.01.06 

2022

Microscopic and biochemical monitoring of endosomal trafficking and extracellular vesicle secretion in an endogenous in vivo modelK Linnemannstöns, P Karuna M, L Witte, D Choezom, M Honemann‐Capito, AS Lagurin, CV Schmidt, S Shrikhande, LK Steinmetz, M Wiebke, C Lenz, JC Gross, Journal of Extracellular Vesicles 11 (9), 12263 |  | 2022

Neutral sphingomyelinase 2 controls exosome secretion by counteracting V-ATPase-mediated endosome acidification
D Choezom, JC Gross, Journal of Cell Science 135 (5), jcs259324 | 15 | 2022

Characterization of two novel neutral sphingomyelinase 2 inhibitors in endosomal sorting and extracellular vesicle biogenesis

D Choezom, JC Gross, Extracellular Vesicles 1 (4), https://doi.org/10.47184/tev.2022.01.02 

2021

The power of imaging to understand extracellular vesicle biology in vivo. Verweij FJ, Balaj L, Boulanger CM, Carter DRF, Compeer EB, D'Angelo G, El Andaloussi S, Goetz JG, Gross JC, Hyenne V, Krämer-Albers EM, Lai CP, Loyer X, Marki A, Momma S, Nolte-'t Hoen ENM, Pegtel DM, Peinado H, Raposo G, Rilla K, Tahara H, Théry C, van Royen ME, Vandenbroucke RE, Wehman AM, Witwer K, Wu Z, Wubbolts R, van Niel G. Nat Methods. 2021 Aug 26. doi: 10.1038/s41592-021-01206-3. Online ahead of print. PMID: 34446922 Review.

Visualization and Quantitation of Wg trafficking in the Drosophila Wing Imaginal Epithelium

Witte L, Linnemannstöns K, Honemann-Capito M, Gross JC, Bio-protocol 11 (11), e4040-e4040

Extracellular WNTs: Trafficking, Exosomes, and Ligand–Receptor Interaction

JC Gross, Pharmacology of the WNT Signaling System, 29-43

2020

Wnt10b-GSK3β-dependent Wnt/STOP signaling prevents aneuploidy in human somatic cells. Lin YC, Haas A, Bufe A, Parbin S, Hennecke M, Voloshanenko O, Gross J, Boutros M, Acebron SP, Bastians H. Life Sci Alliance. 2020 Nov 30;4(1):e202000855. doi: 10.26508/lsa.202000855. Print 2021 Jan. PMID: 33257473

Phosphorylation of Ykt6 SNARE Domain Regulates Its Membrane Recruitment and Activity. Karuna M P, Witte L, Linnemannstoens K, Choezom D, Danieli-Mackay A, Honemann-Capito M, Gross JC. Biomolecules. 2020 Nov 16;10(11):1560. doi: 10.3390/biom10111560. PMID: 33207719

Wie Zellen kommunizieren: extrazelluläre Vesikel als Signalübermittler. Witte L & Gross JC

BIOspektrum volume 26, pages475–477(2020)

Crossroads of the endosomal machinery:  Multivesicular bodies, small extracellular vesicles and autophagy. Parbin S and Gross JC, Trillium Extracellular Vesicles 1(1) 2020

Kinesin motor Klp98A mediates apical to basal Wg transport. Witte L, Linnemannstöns K, Schmitt K., Honemann-Capito M, Grawe F, Wodarz A and Gross JC, Development 2020 dev.186833 doi: 10.1242/dev.186833 Published 14 July 2020

Ykt6-dependent endosomal recycling is required for Wnt secretion in the Drosophila wing epithelium. Linnemannstöns K, Witte L, Karuna P, Kittel J, Danieli A, Müller D, Nitsch L, Honemann-Capito M, Grawe F, Wodarz A, Gross JC. Development. 2020 Jul 1:dev.185421. doi: 10.1242/dev.185421. Online ahead of print

eGFP-tagged Wnt-3a enables functional analysis of Wnt trafficking and signaling and kinetic assessment of Wnt binding to full-length Frizzled. Wesslowski J, Kozielewicz P, Wang X, Cui H, Schihada H, Kranz D, Karuna M P, Levkin P, Gross JC, Boutros M, Schulte G, Davidson G. J Biol Chem. 2020 May 7:jbc.RA120.012892. doi: 10.1074/jbc.RA120.012892. Online ahead of print. PMID: 32381507 

2019

miR-10a-5p and miR-29b-3p as extracellular vesicle-associated prostate cancer detection markers.Worst TS, Previti C, Diessl N, Gross JC, Hoffmann L, Nitschke K, Frey L, Thomas V, Kahlert C, Bieback K, Torres Crigna A, Fricke F, Porubsky S, Westhoff N, von Hardenberg J, Nuhn P, Erben P, Michel MS, Boutros M, Cancers (Basel). 2019 Dec 21;12(1) (IF: 6.2) 

Extracellular Vesicles - Developmental messengers of tissue crosstalk. Witte L and Gross JC, Trillium Extracellular Vesicles 1(1) 2019

2018

Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines. Théry C, Witwer KW, Aikawa E, Alcaraz MJ, Anderson JD, Andriantsitohaina R, Antoniou A,  Arab T, Archer F,  Atkin-Smith GK,   Ayre DC, Bach JM, Bachurski D, Baharvand H, Balaj L,  Baldacchino S,  Bauer NN, Baxter AA, Bebawy M, Beckham C, Zavec AB, Abderrahim Benmoussa A, Berardi AC, Bergese P, Bielska E, Blenkiron C, Bobis-Wozowicz S, Boilard E, Boireau W, Bongiovanni A, Borràs FE, Bosch S, Boulanger CM,  Breakefield X, Breglio AM, Á Brennan M, Brigstock DR, Brisson A, Broekman ML, Bromberg JF, Bryl-Górecka P, Buch S, Buck AH,Burger D, Busatto S, Buschmann D, Bussolati B, Buzás EI,  Byrd JB, Camussi G, Carter DRF, Caruso S, Chamley LW, Chang YT, Chen C, Chen S, Cheng L, Chin AR, Clayton A, Clerici SP, Cocks A,  Cocucci E, Coffey RJ, Cordeiro-da-Silva A, Couch Y, Coumans FAW, Coyle B, Crescitelli R, Ferreira Criado M, D ’Souza-Schorey C, Das S, Chaudhuri AD, de Candia P, De Santana Jr EF, De Wever O,  del Portillo HA, Demaret T, Deville S, Devitt A, Dhondt B, Di Vizio D, Dieterich LC, Dolo V, Dominguez Rubio AP, Dominici M, Dourado MR, Driedonks TAP,  Duarte FV, Duncan HM, Eichenberger RM, Ekström K, El Andaloussi S, Elie-Caille C, Erdbrügger U, Falcón-Pérez JM, Fatima F, Fish JE, Flores-Bellver M, Försönits A,  Frelet-Barrand A, Fricke F, Fuhrmann G, Gabrielsson S, Gámez-Valero A, Gardiner C, Gärtner K, Gaudin R,  Gho YS, Giebel B, Gilbert C, Gimona M, Giusti I, Goberdhan DC, Görgens A, Gorski SM,  Greening DW,  Gross JC, Gualerzi A, Gupta GN, Gustafson D, Handberg A, Haraszti RA, Harrison P, Hegyesi H, Hendrix A, Hill AF, Hochberg FH, Hoffmann KH, Holder B, Holthofer H, Hosseinkhani B, Hu G, Huang Y, Huber V, Hunt S, Gamal-Eldin Ibrahim A, Ikezu T, Inal JM, Isin M, Ivanova A, Jackson HK,  Jacobsen S, Jay SM, Jayachandran M, Jenster G, Lanzhou Jiang, Suzanne M Johnson, Jennifer C Jones,  Jong A, Tijana Jovanovic-Talisman, Stephanie Jung, Raghu Kalluri, Shin-ichi Kano, Kaur S, Kawamura Y,  Keller ET, Khamari D,  Khomyakova E, Khvorova A, Kierulf P, Kim KP,  Kislinger T,  Klingeborn M, Klinke II DJ, Kornek M, Kosanović MM, Kovács AF, Krämer-Albers EM, Krasemann S, Krause M, Kurochkin IV, Kusuma GD, Sören Kuypers S, Saara Laitinen,  Langevin SM, Languino LR,  Lannigan J, Lässer C, Laurent LC, Lavieu G, Lázaro-Ibáñez E,  Le Lay S, Lee MS, Yi Xin Fiona Lee, Debora S Lemos, Lenassi M, Leszczynska A, Isaac TS Li, Ke Liao, Libregts SF, Ligeti E, Lim R, Lim SK, Linē A,  Linnemannstöns K, Llorente A, Lombard CA, Lorenowicz MJ, Lörincz AM, Lötvall J, Lovett J, Lowry MC, Loyer X, Lu Q, Lukomska B, Lunavat TR,  LN Maas S, Malhi H, Marcilla A, Mariani J, Mariscal J, Martens-Uzunova ES, Martin-Jaular L, Martinez MC, Martins VR, Mathieu M, Mathivanan S, Maugeri M, McGinnis LK, Mark J McVey,  Meckes Jr DG,  Meehan KL, Mertens I, Minciacchi VR, Möller A,  Møller Jørgensen M,  Morales-Kastresana A, Morhayim J, Mullier F, Muraca M, Musante L, Mussack V, Muth DC, Myburgh KH,  Najrana T, Nawaz M, Nazarenko I, Nejsum P, Neri C, Neri T,  Nieuwland R, Nimrichter L, Nolan JP, Nolte-’t Hoen EN, Hooten NN,  O’Driscoll L,  O’Grady T, O’Loghlen A, Ochiya T, Olivier M, Ortiz A, Ortiz LA, Xabier Osteikoetxea X, Ostegaard O,  Ostrowski M, Park J, Pegtel MD, Peinado H, Perut F, Pfaffl MW, Phinney DG, Pieters BC, Pink RC, Pisetsky DS, Pogge von Strandmann E, Polakovicova I, Poon IK, Powell BH, Prada I, Pulliam L, Quesenberry P, Radeghieri A, Raffai RL, Raimondo S, Rak J, Ramirez MI, Raposo G, Rayyan MS, Regev-Rudzki N, Ricklefs FL, Robbins PD, Roberts DD, Rodrigues SC, Rohde E, Rome S, Kasper MA Rouschop,Rughetti A, Russell AE, Saá P, Sahoo S, Salas-Huenuleo E, Sánchez C, Saugstad JA, Saul MJ, Schiffelers RM, Schneider R, Hiorth Schøyen T, Scott A, Shahaj E,  Sharma S, Shatnyeva O, Shekari F, Vilas Shelke G, Shetty AK, Shiba K, Siljander PR, Silva AM, Skowronek A, Orman L Snyder II, Soares RP, Sódar BW,  Soekmadji C,  Sotillo J, Stahl PD, Stoorvogel W, Stott SL, Strasser EF,  Swift S, Tahara H, Tewari M, Timms K, Tiwari S, Tixeira R, Tkach M, Toh WS, Tomasini R, Torrecilhas AC, Tosar JP, Toxavidis V, Lorena Urbanelli L, Vader P, van Balkom BW, van der Grein SG, Van Deun J, Martijn JC van Herwijnen, Van Keuren-Jensen K, van Niel G, van Royen ME, Andre J van Wijnen, Vasconcelos HM, Vechetti Jr IJ, Veit TD, Vella LJ, Velot E, Verweij FJ, Vestad B, Viñas JL, Visnovitz T, Vukman KV, Wahlgren J, Watson DC, Wauben MH,  Weaver A, Webber JP, Weber V, Wehman AM, Weiss DJ,  Welsh JA,  Wendt S, Wheelock AM,  Wiener Z, Witte L, Wolfram J, Xagorari A, Xander P, Xu J,  Yan X, Yáñez-Mó M, Yin H, Yuana Y,  Zappulli V, Zarubova J, Žėkas V, Zhang JY,  Zhao Z,  Zheng L, Zheutlin AR, Zickler AM, Zimmermann P, Zivkovic AM,  Zocco D and Zuba-Surma EK (2018) Journal of Extracellular Vesicles. 8(1). (IF: 11.0)

The mingle-mangle of Wnt signaling and Extracellular Vesicles: Functional implications for cardiovascular research. Gross JC and Zelarayan LC, Review. Front Cardiovasc Med. 2018 Feb 22;5:10. doi: 10.3389/fcvm.2018.00010. eCollection 2018. 

2017

Neutral sphingomyelinases control Extracellular Vesicles budding from the plasma membrane. Menck K, Sönmezer C, Worst T, Schulze M, Dihazi G, Streit F, Erdmann G, Kling S, Boutros M, Binder C and Gross JC. Journal of Extracellular Vesicles Vol. 6, Iss. 1,2017

A database-augmented, exosome-based mass spectrometry approach exemplarily identifies circulating claudin 3 as biomarker in prostate cancer. Worst TS, von Hardenberg J, Gross JC, Erben P, Schnölzer M, Hausser I, Bugert P, Michel MS & Boutros M. MCP 2017 Jun;16(6):998-1008. doi: 10.1074/mcp.M117.068577. Epub  2017 Apr 9. (IF:5.9)

2016

Cryptochrome 1 in Retinal Cone Photoreceptors Suggests a Novel Functional Role in Mammals. Nießner C, Denzau S, Malkemper EP, Gross JC, Burda H, Winklhofer M, Peichl L. Sci Rep. 2016 Feb22;6:21848. doi: 10.1038/srep21848. PMID: 26898837. (IF: 5.2)

Biochemical Methods to Analyze Wnt Protein Secretion. Glaeser K, Boutros M, Gross JC. Methods Mol Biol.2016;1481:17-28. doi: 10.1007/978-1-4939-6393-5_3. PMID: 27590148.

Seasonally Changing Cryptochrome 1b Expression in the Retinal Ganglion Cells of a Migrating Passerine Bird. Nießner C, Gross JC, Denzau S, Peichl L, Fleissner G, Wiltschko W, Wiltschko R. PLoS One. 2016 Mar 8;11(3):e0150377. doi:10.1371/journal.pone.0150377. PMID: 26953690 (IF: 4.4)

2015

EVpedia: a community web portal for extracellular vesicles research. Kim DK, Lee J, Kim SR, Choi DS, Yoon YJ, Kim JH, Go G, Nhung D, Hong K, Jang SC, Kim SH, Park KS, Kim OY, Park HT, Seo JH, Aikawa E, Baj-Krzyworzeka M, van Balkom BW, Belting M, Blanc L, Bond V, Bongiovanni A, Borràs FE, Buée L, Buzás EI, Cheng L, Clayton A, Cocucci E, Dela Cruz CS, Desiderio DM, Di Vizio D, Ekström K, Falcon-Perez JM, Gardiner C, Giebel B, Greening DW, Gross JC, Gupta D, Hendrix A, Hill AF, Hill MM, Nolte-'t Hoen E, Hwang DW, Inal J, Jagannadham MV, Jayachandran M, Jee YK, Jørgensen M, Kim KP, Kim YK, Kislinger T, Lässer C, Lee DS, Lee H, van Leeuwen J, Lener T, Liu ML, Lötvall J, Marcilla A, Mathivanan S, Möller A, Morhayim J, Mullier F, Nazarenko I, Nieuwland R, Nunes DN, Pang K, Park J, Patel T, Pocsfalvi G, Del Portillo H, Putz U, Ramirez MI, Rodrigues ML, Roh TY, Royo F, Sahoo S, Schiffelers R, Sharma S, Siljander P, Simpson RJ, Soekmadji C, Stahl P, Stensballe A, Stępień E, Tahara H, Trummer A, Valadi H, Vella LJ, Wai SN, Witwer K, Yáñez-Mó M, Youn H, Zeidler R, Gho YS. Bioinformatics. 2015 Mar 15;31(6):933-9.doi: 10.1093/bioinformatics/btu741. PMID: 25388151 (IF:5.7)

2014 - 2009

Molecular dissection of Wnt3a-Frizzled8 interaction reveals essential and modulatory determinants of Wnt signaling activity. Kumar S, Žigman M, Patel TR, Trageser B, Gross JC, Rahm K, Boutros M, Gradl D, Steinbeisser H, Holstein T, Stetefeld J, Özbek S. BMC Biol. 2014 May 30;12:44. doi:10.1186/1741-7007-12-44. PMID: 24885675 (IF: 6.9)

Induction and transport of Wnt 5a during macrophage-induced malignant invasion is mediated by two types of extracellular vesicles. Menck K, Klemm F, Gross JC, Pukrop T, Wenzel D, Binder C. Oncotarget. 2013 Nov;4(11):2057-66. PMID: 24185202 (IF: 6.6)

Loss of epidermal Evi/Wls results in a phenotype resembling psoriasiform dermatitis. Augustin I, Gross J, Baumann D, Korn C, Kerr G, Grigoryan T, Mauch C,Birchmeier W, Boutros M. J Exp Med. 2013Aug 26;210(9):1761-77. doi: 10.1084/jem.20121871. PMID: 23918954 (IF: 13.9)

Secretion and extracellular space travel of Wnt proteins. Gross JC, Boutros M. Curr Opin Genet Dev.2013 Aug;23(4):385-90. doi: 10.1016/j.gde.2013.02.017. Review. PMID: 23540564. (IF: 5.9)

Active Wnt proteins are secreted on exosomes. Gross JC, Chaudhary V, Bartscherer K, Boutros M. Nat Cell Biol. 2012 Oct;14(10):1036-45. doi:10.1038/ncb2574. PMID: 22983114. (IF: 20.7)

Avian ultraviolet/violet cones identified as probable magnetoreceptors. Niessner C, Denzau S, Gross JC, Peichl L, Bischof HJ, Fleissner G, Wiltschko W, Wiltschko R. PLoS One.2011;6(5):e20091. doi:10.1371/journal.pone.0020091. PMID: 21647441 (IF: 4.0)

Interactions of plakoglobin and beta-catenin with desmosomal cadherins: basis of selective exclusion of alpha-and beta-catenin from desmosomes. Choi HJ, Gross JC, Pokutta S, Weis WI. J Biol Chem.2009 Nov 13;284(46):31776-88. doi:10.1074/jbc.M109.047928. PubMed PMID: 19759396 (IF: 5.3)

E-cadherin surface levels in epithelial growth factor-stimulated cells depend on adherens junction protein shrew-1. Gross JC, Schreiner A, Engels K, Starzinski-Powitz A. Mol Biol Cell. 2009 Aug;20(15):3598-607. doi:10.1091/mbc.E08-12-1240. PMID: 19515834 (IF: 5.9)